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Baseline shows continuous increase in response even with buffer injection
- Madhu
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1 month 2 weeks ago #1
by Madhu
Baseline shows continuous increase in response even with buffer injection was created by Madhu
Recently in T200 we are facing a problem with all established assay and its irrespective of chemistry or protein.
There is continuous increasing drift in the baseline for every cycle of injection, initially we doubted on protein aggregation, regeneration but this happens irrespective of chip surface, protein interaction.
Even a buffer injection is causing the increase in the baseline continually, currently we are trying to check if its the CM5 matrix issue or the instrument issue. Meanwhile all the system check shows pass result .
Anyone seen this kind of issue or any input for resolving please respond
thank you
There is continuous increasing drift in the baseline for every cycle of injection, initially we doubted on protein aggregation, regeneration but this happens irrespective of chip surface, protein interaction.
Even a buffer injection is causing the increase in the baseline continually, currently we are trying to check if its the CM5 matrix issue or the instrument issue. Meanwhile all the system check shows pass result .
Anyone seen this kind of issue or any input for resolving please respond
thank you
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- Arnoud
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4 weeks 2 days ago #2
by Arnoud
Replied by Arnoud on topic Baseline shows continuous increase in response even with buffer injection
You say the system check is ok, does that mean that there is no drift during the system check?
What if you try a different buffer?
What if you try a different buffer?
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