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Weekly quiz

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Question: how much ligand should you immobilize for analyte concentration measurements?

A: intermediate
B: high
C: low to intermediate
D: low

    • kinetic relation

      Is the equilibrium dissociation constant all what matters?

      In kinetics there seems to be a race for the best (lowest in this context) equilibrium dissociation constant (KD) there is. Interactions are compared by their equilibrium dissociation constant and that is it. Similarly often only the IC50 of a system is given while the full kinetics are not well established.

      In my opinion, reporting a single value for a kinetic interaction is hiding all the other information present in the measurements between two or more components.
    • NanoTemper logo

      Improving protein quality

      With regular maintenance your instrument is in perfect order. In addition you make the best, clean and degassed buffers. But what about the proteins you use? Are they of the best quality? And this new batch is the same as the previous, you are sure of that?
      Is your protein affected by the buffer and immobilization conditions used? Your assay problems might be caused by poor protein quality!
      Luckily there is an easy and fast way to check the integrity of your proteins. Nanotemper Technologies provides various instruments to check your protein quality, all using a minimum of sample. .
    • Digital Art

      Digital SPR by Nicoya

      Nicoya, a leading provider of advanced analytical instruments for the biotechnology and pharmaceutical industries, today announced the launch of Alto, the world’s first fully-automated, high-throughput benchtop surface plasmon resonance (SPR) system. As the first-ever SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology, Alto provides high quality, label-free interaction analysis.
    • SPR is not an Art

      SPR is not an Art!

      Surface Plasmon Resonance (SPR) seems to be difficult for many people. Judging on the literature many of you are struggling to get nice sensorgrams with easy to fit curves. However, SPR is straightforward when you know what you are doing. Consequently, you have to learn how SPR works, how to perform an experiment and how to analyze it. So what are the key parts of a proper SPR experiment?
    • Digital Art

      Blog of Ryan Denomme

      Ryan Denomme of Nicoya publishes a nice blog about SPR and related topics. For instance about preserving precious sample by optimizing preconcentration and immobilization or what the difference is between SPR and LSPR.
      Definitely worthwhile to check this blog!


The KD assistant is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration. The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly. Goto Software


The SPR-forum is dedicated to the combination between SPR and biomolecular interactions. There are many questions asked and answered so browse through the topics.
Feel free to post your questions about the technology, instruments or experimental set-up.


The BiaMethodWriter is a program to write the automation methods in a more interactive way. This program uses pop up menus and dialog boxes to define the values of the parameters. Remembering these odd parameters is not necessary any more.
It works for the control software with the Biacore 2000/3000. Is not compatabile with the new T200 control software.

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clean immobilization stabilization association steady state dissociation regeneration analysis