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Weekly quiz

Ligand capture

Question: The capture approach of a ligand leads to …

A: a more homogenous ligand surface than direct immobilization
B: less mass-transport than direct immobilization
C: a higher ligand density on the sensor surface
D: a more stable ligand surface

    • NanoTemper logo

      Improving protein quality

      With regular maintenance your instrument is in perfect order. In addition you make the best, clean and degassed buffers. But what about the proteins you use? Are they of the best quality? And this new batch is the same as the previous, you are sure of that?
      Is your protein affected by the buffer and immobilization conditions used? Your assay problems might be caused by poor protein quality!
      Luckily there is an easy and fast way to check the integrity of your proteins. Nanotemper Technologies provides various instruments to check your protein quality, all using a minimum of sample. .
    • Digital Art

      Digital SPR by Nicoya

      Nicoya, a leading provider of advanced analytical instruments for the biotechnology and pharmaceutical industries, today announced the launch of Alto, the world’s first fully-automated, high-throughput benchtop surface plasmon resonance (SPR) system. As the first-ever SPR instrument to integrate digital microfluidics (DMF), artificial intelligence (AI), and nanotechnology, Alto provides high quality, label-free interaction analysis.
    • SPR is not an Art

      SPR is not an Art!

      Surface Plasmon Resonance (SPR) seems to be difficult for many people. Judging on the literature many of you are struggling to get nice sensorgrams with easy to fit curves. However, SPR is straightforward when you know what you are doing. Consequently, you have to learn how SPR works, how to perform an experiment and how to analyze it. So what are the key parts of a proper SPR experiment?
    • Digital Art

      Blog of Ryan Denomme

      Ryan Denomme of Nicoya publishes a nice blog about SPR and related topics. For instance about preserving precious sample by optimizing preconcentration and immobilization or what the difference is between SPR and LSPR.
      Definitely worthwhile to check this blog!

    • LowFigure

      How Low Can You Go ?

      In SPR experiments, low responses are preferred over high responses. This is because high responses in general are affected by mass transport and non 1:1 interactions. But how low can you go?
      This depends in the first place on the instrument you have chosen. The signal should be above the noise level of the baseline. The curves should be easily recognisable and replicates should overlay.

KD-assistant

The KD assistant is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration. The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly. Goto Software

SPR-Forum

The SPR-forum is dedicated to the combination between SPR and biomolecular interactions. There are many questions asked and answered so browse through the topics.
Feel free to post your questions about the technology, instruments or experimental set-up.

BiaMethodWriter

The BiaMethodWriter is a program to write the automation methods in a more interactive way. This program uses pop up menus and dialog boxes to define the values of the parameters. Remembering these odd parameters is not necessary any more.
It works for the control software with the Biacore 2000/3000. Is not compatabile with the new T200 control software.
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Your Help

Your help is appreciated!. Give your feedback about this website, tips for better experiments or report interesting links. Maybe you have nice tips for every body or you can contribute in a more substantial way to the SPR-pages. Check out the forum for questions and answers.
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Web links

Look at the many books, suppliers, machines, software and other links!? Did you look at the FAQ and download pages? You will find a lot of interesting stuff there.
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Donate

The SPRpages are not affiliated to any company or institute. For the maintenance of the SPRpages we rely on the donations and the sales of the SPRpage book and the SPR-simulation program. Therefore, any donation is appreciated.

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clean immobilization stabilization association steady state dissociation regeneration analysis