ProteOn sensor chips

Bio-Rad has discontinued the production of the ProteOn XPR36 and its sensor chips. XanTec offers now sensor chips which are compatible with the ProteOn XPR36 system. Both 2D and 3D hydrogel surfaces with different modifications and functionalities are available. The table below shows XanTec's equivalents to the Bio-Rad sensor chips.

ProteOn sensor chips by Xantec
Modification Bio-Rad XanTec
short matrix, normal capacity GLC CMD50L, HC30M
Alternative: AL (alginate surface)
normal matrix, low capacity HLC200M
normal matrix, normal capacity GLM CMD200M, CMD500L, HC200M
normal matrix, high capacity CMD700M, HC1000M
long matrix, high capacity GLH HC1500M
no matrix, low capacity CMDP / HCP
poly HIS binding group HTG / HTE High affinity: NiHC1500M, NiHC200M
Standard affinity: NiD200M
hydrophobic surface HPP
lipid capturing surface GLC + lipid kit LD (hydrogel) & LP (2D)
lipid capturing surface (DNA based) LCP
biotin capturing surface NLC Streptavidin: SAD200M & others
Neutravidin: NAHC200M & others
biotin capture surface (DNA based)
biotin capture surface (HIS-tag based) HTG / HTE + HIS-Streptavidin High affinity: NiHC1500M, NiHC200M
Standard affinity: NiD200M
plain gold surface AU
click chemistry Azide surface: AZHC200M & others
DBCO surface: DCD200M & others
zwitterionic surface ZC30M, ZC80M, ZC150D
streptavidin capture surface BHC30M, BD200M & others
Xantec sensor chips selection guide

ProteOn sensor chips (discontinued)

ProteOn sensor chips are prepared with a modified alginate polymer bound to a gold surface. The alginate layer is attached in different structures to provide surfaces with different binding capacities. The hydrophilic nature of the alginate layer creates a solution-like environment that prevents denaturation of the immobilized ligand and nonspecific adsorption of the analyte. The carboxyl groups on the alginate molecule are designed for easy activation with the ProteOn amine coupling kit using proprietary technology. Because of the increased activation yield on the ProteOn sensor chips the alginate layer is thinner than the dextran layer of other sensor chip types. In fact the GLC sensor chip layer thickness is close to 10 nm.
ProteOn sensor chips provide well-characterized surface chemistries optimized to control ligand immobilization densities and maintain high ligand activities suitable for detailed kinetic analysis as well as qualitative screening applications. Each ProteOn sensor chip is enclosed in a bar-coded cartridge for data tracking and archiving (1).

Sensor chip comparison
modification Biacore Bio-Rad
short matrix, normal capacity CM3 GLC
normal matrix, low capacity CM4
normal matrix, normal capacity CM5 GLM
normal matrix, high capacity CM7
long matrix, high capacity GLH
no matrix, low capacity C1
poly HIS binding group NTA HTG / HTE
hydrophobic surface HPA
lipid capturing surface L1 GLC + lipid kit
lipid capturing surface (DNA based) LCP
biotin capturing surface SA NLC
biotin capture surface (DNA based) CAPture kit
biotin capture surface (HIS-tag based) HTG / HTE + HIS-Streptavidin
plain gold surface AU


(1)Bio-Rad Bio-Rad Website. (2012).


  1. ProteOn XPR 36 is not sold any more