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Problems with relative response (very hi
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Problems with relative response (very high baseline and very high fc2-fc1 differ

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1 week 5 days ago #1 by JB
Hello everyone! 
The Biacore X100 device has not been launched for many years. When it was used (together with the company engineer), carbohydrates were driven on chips with immobilized carbohydrate-binding protein. Judging by the report files, the system check and pump calibration procedure was carried out several times (all times successfully), but after that the shutdown procedure was performed, after which the device was no longer used. Today, when turning on the device and the control software, the system offered to flush the system with HBS-EP+ buffer and system check and pump calibration. When performing system check and pump calibration (1x HBS-EP+ buffer, 15% BIAtest sucrose solution, new CM5 chip), we received several errors. So, we have a very high buffer baseline, in FC1 - 22,000 RU, FC2 - 55,000 RU, FC2-FC1 - 35,000 RU. When injecting 15% sucrose, we get a response in FC1 of 21-22,000 RU, and in FC2 - 7-8,000 RU. After that, we performed the desorb and sanitize procedure (BIAmaintenance kit), then the device worked in standby mode for 3 hours (following the recommendations). After that, we repeated the system check and pump calibration procedure on the CM5 chip. We got approximately the same results as the first time. We have problems with the relative response indicator for FC2-FC1, and also problems with noise (RU/s)
All solutions were prepared, filtered (0.22 µm) and degassed immediately before use. What can you suggest? I am a newbie in SPR, I would like to hear recommendations from experienced users.

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1 week 5 days ago #2 by Arnoud
Can you post the last sensorgram and the report for inspection?

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