This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
Inconsistent KD
- rizwan007
- Topic Author
- Offline
- Banned
-
5 years 2 months ago #1
by rizwan007
Inconsistent KD was created by rizwan007
Hi am trying to develop a method for FcRn and am observing that KD is varying a lot within the same run. 6 sets of the same analyte was run in a single run with the same preparation to check the variability. Have observed the KD varying From 53 nM to 350 nM . Could you please suggest what has to be done?
Please Log in or Create an account to join the conversation.
- Arnoud
- Moderator
-
5 years 2 months ago #2
by Arnoud
Replied by Arnoud on topic Inconsistent KD
The numbers say that the replicates are not very well. But as told before, don't rank/judge your experiments on the KD alone!
Please give some more information. ka, kd etc, Sensorgrams, fittings...
Arnoud
Please give some more information. ka, kd etc, Sensorgrams, fittings...
Arnoud
Please Log in or Create an account to join the conversation.
Moderators: Arnoud, Arnoud