These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
NSB reducer
- OldForum
- Topic Author
- Visitor
-
12 years 7 months ago #1
by OldForum
NSB reducer was created by OldForum
Hello. Has anyone used the NSB reducer from Biacore (10mg/mL of carboxymethyl dextran in 0.15M NaCL)? It said to use a 1:10 diln with the samples before injection (comes in a 10mg/mL solution). How about the buffer? Shouldn't that contain CM dextran as well to match sample and buffer matrix? I was thinking of just preparing the HBS buffer and add 1mg/mL of CM-dextran (powder), filter and then use this buffer to prepare the samples. Kindly comment. Thanks.
mtarca
mtarca
Please Log in or Create an account to join the conversation.
- OldForum
- Topic Author
- Visitor
-
12 years 7 months ago #2
by OldForum
Replied by OldForum on topic NSB reducer
Hi mtarca,
I would add it to the flow buffer to make the buffer jumps lower.
I think you should use the lowest concentration which is working for you.
In addition, maybe you should try to identify the source of the non-specific binding.
Arnoud
I would add it to the flow buffer to make the buffer jumps lower.
I think you should use the lowest concentration which is working for you.
In addition, maybe you should try to identify the source of the non-specific binding.
Arnoud
Please Log in or Create an account to join the conversation.