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Tannin binding
- OldForum
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12 years 8 months ago #1
by OldForum
Tannin binding was created by OldForum
Hi,
I'm binding procyanidins (Tannin) to the gold surface, but no success in removing. I've tried with Urea 6M, NaOH 0,1M-Triton 1%, Hydrogen Peroxide. Any suggestions?
John
I'm binding procyanidins (Tannin) to the gold surface, but no success in removing. I've tried with Urea 6M, NaOH 0,1M-Triton 1%, Hydrogen Peroxide. Any suggestions?
John
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- OldForum
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12 years 8 months ago #2
by OldForum
Replied by OldForum on topic Tannin binding
Hi this not be easy because gold adsorbs proteins and other stuff very easy.
Try 4% SDS.
The mixture of 0.1 M NaOH and 1% Triton X-100 should work in most cases but maybe is your protein sticky.
Look at the following link ( www.ti.com/lit/an/slya018a/slya018a.pdf ) for more information and hints.
Try 4% SDS.
The mixture of 0.1 M NaOH and 1% Triton X-100 should work in most cases but maybe is your protein sticky.
Look at the following link ( www.ti.com/lit/an/slya018a/slya018a.pdf ) for more information and hints.
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