These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
DNA-SH immobilisation
- OldForum
- Topic Author
- Visitor
-
13 years 1 month ago #1
by OldForum
DNA-SH immobilisation was created by OldForum
could anyone indicatea protocol for DNA-SH immo onto gold surface ?
thanks
dd
thanks
dd
Please Log in or Create an account to join the conversation.
- OldForum
- Topic Author
- Visitor
-
13 years 1 month ago #2
by OldForum
Replied by OldForum on topic DNA-SH immobilisation
Why would you want to immobilize the SH-DNA directly to the gold surface?
Ik think that using a dextran coated chip and a thiol immobilizing protocol will be more efficient.
Arnoud
Ik think that using a dextran coated chip and a thiol immobilizing protocol will be more efficient.
Arnoud
Please Log in or Create an account to join the conversation.
- OldForum
- Topic Author
- Visitor
-
13 years 1 month ago #3
by OldForum
Replied by OldForum on topic DNA-SH immobilisation
Just dilute the thiolated DNA (~1uM) in a buffer (I use PBS) and incubate it over a couple of hours. Then, if you want, do an extra half hour long incubation of MCH (1mM) in the dark.
Please Log in or Create an account to join the conversation.