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Glycerol in main buffer
- OldForum
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16 years 7 months ago #1
by OldForum
Glycerol in main buffer was created by OldForum
Listen, is it true that glycerol (at 3% v/v concentration) in main buffer (and in samples of analyte) may make the experiment useless as there would not be any signals from eg protein-sugar interactions?
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- OldForum
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16 years 7 months ago #2
by OldForum
Replied by OldForum on topic Glycerol in main buffer
There are papers where 6.5% (w/v) glycerol is added to the running buffer (main buffer?) and the sensorgrams do still lock beautiful so that should not be a problem. In this case small molecules (500Da) are measured with "drug-like properties" (aromatic ring systems) that do have a higher impact on the refractive index = signal than e.g. sugar moieties.
Just dilute your analyte stock 1000-fold in running buffer to eliminate any buffer differences between stock and running buffer that could lead to large bulk effects which overlay your signal.
Strada
Just dilute your analyte stock 1000-fold in running buffer to eliminate any buffer differences between stock and running buffer that could lead to large bulk effects which overlay your signal.
Strada
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