These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
small molecule immobilisation
- OldForum
- Topic Author
- Visitor
-
16 years 7 months ago #1
by OldForum
small molecule immobilisation was created by OldForum
Hi!
I`m trying to immobilize a MW 500 molecule(NHS active ester) on a modified (bearing free amine groups) sensor surface. Has anyone experience in this field?
I would be deeply grateful to any reply concerning this topic.
Pharmchem
I`m trying to immobilize a MW 500 molecule(NHS active ester) on a modified (bearing free amine groups) sensor surface. Has anyone experience in this field?
I would be deeply grateful to any reply concerning this topic.
Pharmchem
Please Log in or Create an account to join the conversation.
- OldForum
- Topic Author
- Visitor
-
16 years 7 months ago #2
by OldForum
Replied by OldForum on topic small molecule immobilisation
Hi,
I did it once the other way round. Attach a NH2 bearing small molecule to the activated ester on the chip. A 1 mM concentration of the small molecule (check solubility!) worked fine in a HBS-N buffer system at 5ul/min with a 10-20 min. injection. The immobilization level was generally in the range of 100-200 RU. Depending on the later experiment u might have to reduce the injection time to reduce the surface capacity. I guess it should work in your set-up as well.
greetz
Strada
I did it once the other way round. Attach a NH2 bearing small molecule to the activated ester on the chip. A 1 mM concentration of the small molecule (check solubility!) worked fine in a HBS-N buffer system at 5ul/min with a 10-20 min. injection. The immobilization level was generally in the range of 100-200 RU. Depending on the later experiment u might have to reduce the injection time to reduce the surface capacity. I guess it should work in your set-up as well.
greetz
Strada
Please Log in or Create an account to join the conversation.