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Direct immobilization
- OldForum
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11 years 4 months ago #1
by OldForum
Direct immobilization was created by OldForum
Hello.
I have always used capture immobilization. It is the first time I'm going to do a Direct immobilization on a CM5 chip. Will use Amine coupling. My Ligand mol. wt. is ~28000. I want to have an RL of approx. 200 RU. Any suggestions on; 1) concentration of ligand to start? 30 ug/mL:? 2) Suggested contact time for the ligand; 3) Suggested time for NHS/EDC activation? 4) How long to block with Ethanolamine. Kindly comment. Thanks.
mtarca
I have always used capture immobilization. It is the first time I'm going to do a Direct immobilization on a CM5 chip. Will use Amine coupling. My Ligand mol. wt. is ~28000. I want to have an RL of approx. 200 RU. Any suggestions on; 1) concentration of ligand to start? 30 ug/mL:? 2) Suggested contact time for the ligand; 3) Suggested time for NHS/EDC activation? 4) How long to block with Ethanolamine. Kindly comment. Thanks.
mtarca
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- OldForum
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11 years 4 months ago - 9 years 2 months ago #2
by OldForum
Replied by OldForum on topic Direct immobilization
Hi,
Did you read the pages www.sprpages.nl/immobilization.html ?
1) start with a pre-concentration experiment and 10 - 30 g/ml
2) depends on the immobilization speed
3) start with 7 minutes
4) 7 minutes
Did you read the pages www.sprpages.nl/immobilization.html ?
1) start with a pre-concentration experiment and 10 - 30 g/ml
2) depends on the immobilization speed
3) start with 7 minutes
4) 7 minutes
Last edit: 9 years 2 months ago by .
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