With regular maintenance your instrument is in perfect order. In addition you make the best, clean and degassed buffers. But what about the proteins you use? Are they of the best quality? And this new batch is the same as the previous, you are sure of that?
Is your protein affected by the buffer and immobilization conditions used? Your assay problems might be caused by poor protein quality!
Luckily there is an easy and fast way to check the integrity of your proteins. Nanotemper Technologies provides various instruments to check your protein quality, all using a minimum of sample. .