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SensorgramSurface Plasmon Resonance (SPR) is a powerful technique to measure biomolecular interactions in real-time in a label free environment. While one of the interactants is immobilized to the sensor surface, the other are free in solution and passed over the surface. Association and dissociation is measured in arbitrary units and displayed in a graph called the sensorgram.
Biomolecular Interaction Analysis is not limited to proteins. Interactions between DNA - DNA, DNA - protein, lipid - protein and hybrid systems of biomolecules and non-biological surfaces can be investigated.
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KD Assistant

The KD assistant is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration. The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly. Goto Software

New on the SPR-Pages

There is a new chapter on the SPR-Pages. Look at Sensorgrams and learn all the important parts of a sensorgram. Learn how to distinguish a good from a bad sensorgram. How to optimize your sensorgram. In addition, learn how to report your result in such a way that everyone believes that you did your experiments properly. Finish this chapter with a quiz!


SPR-Pages book

All the information of the SPR-Pages and more in one book. The book contains the theory and the practical approach of preparing samples and sensor chips, performing the experiment and analyzing the results.
In addition, the book has more background information and experimental strategies are explained. The book contains more in-depth information than the website.
This is a valuable resource near your SPR-instrument!



BiaMethodWriter is a program to write the Biacore 2000 and 3000 automation methods in a more interactive way. The program uses pop up menus and dialog boxes to fill in the different parts. Remembering these odd parameters is not necessary any more.

The program calculates vial and buffer volumes and estimates the total runtime. The report function creates an overview of the method and a vial layout with required volumes. Colored text formatting gives a better overview of the method and makes it easier to find errors. read more ...



clean immobilization stabilization association steady state dissociation regeneration analysis

Your help

Your help is appreciated!. Give your feedback about this website, tips for better experiments or report interesting links. Maybe you have nice tips for every body or you can contribute in a more substantial way to the SPR-pages.
Check out the forum for questions and answers.

Web links

Look at the weblinks with other label free techniques, many books, suppliers, machines, software and other links!?
Did you look at the FAQ and download pages? You will find a lot of interesting stuff there.

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SPRpages book


The book contains more additional information and figures compared to the SPR-Pages. In addition, the book can serve as a reference next to your SPR machine. By buying this book you directly support the SPRpages.

Springer et al introduces single surface referencing. A single functionalized surface is split into the detection and reference channel to which complex sample (detection) and complex sample mixed with biomolecules binding to the analyte and thus inhibiting the binding of the analyte to the functionalized surface (reference) is introduced. Goto reference...
April 23-24, 2014 Hilton, San Diego, CA
Cambridge Healthtech Institute’s seventh annual Protein-Protein Interactions meeting will highlight the different disease areas where targeting PPIs is showing promise and will discuss lead optimization challenges for the medicinal chemist.
Goto website...
4-12 August, 2014 - Wellcome Trust Genome Campus, Hinxton, Cambridge, UK. This popular lab-based course provides training in the latest laboratory techniques and bioinformatics tools necessary to discover protein interactions and understand protein function.
Goto Website ...
The 26th Anniversary World Congress on Biosensors will take place 25-27 may 2016 at Svenska Massan, the Swedish Exhibition and Congress centre in Gothenburg.
Take a look at the venue.

June 1 – 4, 2014 La Jolla, California
Join leading scientists at the Developments in Protein Interaction Analysis (DiPIA) conference and discover the latest applications, results, and troubleshooting tips in biomolecular interaction and stability analyses.
Goto website...
Zhou et al. developed a gravity driven SPR sensor. This power-free flow injection system is easy to use and the internal reference compensates for flow fluctuations. The miniature and power-free flow injection system would open the possibility to build a miniature system for point of care testing. Goto referenence...
26-30 May 2014 in Melbourne Convention and Exhibition Centre, Australia. Biosensors 2014 is a international melting pot of expertise, developments, research and commercial opportunity – attracting attendees from both the academic and commercial sectors for maximum knowledge exchange. Goto website...



Making programming methods easier.



This well designed program helps you to clean and analyse your sensorgrams in minutes. Reference subtraction, double referencing, DMSO compensation, almost with one mouse click. Version 2c of Scrubber extends the program with kinetic models for simple binding (association and dissociation rates) and a model for mass transport limited binding. Info