In SPR experiments, low responses are preferred over high responses. This is because high responses in general are affected by mass transport and non 1:1 interactions. But how low can you go?
This depends in the first place on the instrument you have chosen. The signal should be above the noise level of the baseline. The curves should be easily recognisable and replicates should overlay.
Although the phenomenon of surface plasmon resonance (SPR) was known for a long time (see Wood; 1902), in 1990 Pharmacia Biosensor AB brought the first commercial SPR instrument to the market. For a long time, these so-called Biacore instruments set the standard in research using SPR. Nowadays we say that we do a Biacore experiment when we mean that we do a SPR experiment. Since then, the instruments have been evolved and several new manufacturers are selling SPR instruments with new features (SPR imaging: IBIS; Interaction Array: Bio-Rad; multi-parametric SPR: BioNavis ), new sensor chip types (alginate surface: Bio-Rad; hydrophilic SAM: Xantec; graphene surface: Sensia) and software wizards to guide the user.
The instruments are all in best shape and performing excellent. However, how is the user doing? Has he also developed with the instruments or is he struggling to keep up?Read more on 25 years of development
The KD assistant is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration. The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly. Goto Software
There is a new chapter on the SPR-Pages. Look at Sensorgrams and learn all the important parts of a sensorgram. Learn how to distinguish a good from a bad sensorgram. How to optimize your sensorgram. In addition, learn how to report your result in such a way that everyone believes that you did your experiments properly. Finish this chapter with a quiz!
All the information of the SPR-Pages and more in one book. The book contains the theory and the practical approach of preparing samples and sensor chips, performing the experiment and analyzing the results.
In addition, the book has more background information and experimental strategies are explained. The book contains more in-depth information than the website.
This is a valuable resource near your SPR-instrument!
BiaMethodWriter is a program to write the Biacore 2000 and 3000 automation methods in a more interactive way. The program uses pop up menus and dialog boxes to fill in the different parts. Remembering these odd parameters is not necessary any more.
The program calculates vial and buffer volumes and estimates the total runtime. The report function creates an overview of the method and a vial layout with required volumes. Colored text formatting gives a better overview of the method and makes it easier to find errors. read more ...
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The book contains more additional information and figures compared to the SPR-Pages. In addition, the book can serve as a reference next to your SPR machine. By buying this book you directly support the SPRpages.
Making programming methods easier.
This well designed program helps you to clean and analyse your sensorgrams in minutes. Reference subtraction, double referencing, DMSO compensation, almost with one mouse click. Version 2c of Scrubber extends the program with kinetic models for simple binding (association and dissociation rates) and a model for mass transport limited binding.