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How low can you go?

In SPR experiments, low responses are preferred over high responses. This is because high responses in general are affected by mass transport and non 1:1 interactions. But how low can you go?
This depends in the first place on the instrument you have chosen. The signal should be above the noise level of the baseline. The curves should be easily recognisable and replicates should overlay.

Read more on low responses

25 years of SPR

Although the phenomenon of surface plasmon resonance (SPR) was known for a long time (see Wood; 1902), in 1990 Pharmacia Biosensor AB brought the first commercial SPR instrument to the market. For a long time, these so-called Biacore instruments set the standard in research using SPR. Nowadays we say that we do a Biacore experiment when we mean that we do a SPR experiment. Since then, the instruments have been evolved and several new manufacturers are selling SPR instruments with new features (SPR imaging: IBIS; Interaction Array: Bio-Rad; multi-parametric SPR: BioNavis ), new sensor chip types (alginate surface: Bio-Rad; hydrophilic SAM: Xantec; graphene surface: Sensia) and software wizards to guide the user.

The instruments are all in best shape and performing excellent. However, how is the user doing? Has he also developed with the instruments or is he struggling to keep up?

Read more on 25 years of development

KD Assistant

The KD assistant is a small program to assist with the optimization of kinetics runs. The program can calculate the maximal analyte concentration to be used from one manual analyte injection. The input consists of the initial binding rate (slope), the response of the analyte injection and the analyte concentration. The program deals also with the capturing level of the ligand and adjusts the analyte concentration accordingly. Goto Software

New on the SPR-Pages

There is a new chapter on the SPR-Pages. Look at Sensorgrams and learn all the important parts of a sensorgram. Learn how to distinguish a good from a bad sensorgram. How to optimize your sensorgram. In addition, learn how to report your result in such a way that everyone believes that you did your experiments properly. Finish this chapter with a quiz!

 

SPR-Pages book

All the information of the SPR-Pages and more in one book. The book contains the theory and the practical approach of preparing samples and sensor chips, performing the experiment and analyzing the results.
In addition, the book has more background information and experimental strategies are explained. The book contains more in-depth information than the website.
This is a valuable resource near your SPR-instrument!

Info

BiaMethodWriter

BiaMethodWriter is a program to write the Biacore 2000 and 3000 automation methods in a more interactive way. The program uses pop up menus and dialog boxes to fill in the different parts. Remembering these odd parameters is not necessary any more.

The program calculates vial and buffer volumes and estimates the total runtime. The report function creates an overview of the method and a vial layout with required volumes. Colored text formatting gives a better overview of the method and makes it easier to find errors. read more ...

 

SelectGraph

clean immobilization stabilization association steady state dissociation regeneration analysis

Your help

Your help is appreciated!. Give your feedback about this website, tips for better experiments or report interesting links. Maybe you have nice tips for every body or you can contribute in a more substantial way to the SPR-pages.
Check out the forum for questions and answers.

Web links

Look at the many books, suppliers, machines, software and other links!?
Did you look at the FAQ and download pages? You will find a lot of interesting stuff there.

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SPRpages book

SPRbookfigure

The book contains more additional information and figures compared to the SPR-Pages. In addition, the book can serve as a reference next to your SPR machine. By buying this book you directly support the SPRpages.
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OpenSPR-XT

Kitchener, ON, January 20, 2016: Nicoya Lifesciences, a leader in label-free molecular analysis, announced today its newest product, OpenSPR-XT - a fully automated molecular analysis instrument based on the highly successful OpenSPR instrument.

The OpenSPR line of surface plasmon resonance instruments uses proprietary nanotechnology based sensors to provide high quality molecular interaction data at a fraction of the cost of traditional SPR instruments.

More information can be found at Nicoya Lifesciences - OpenSPR-XT

Gravity driven SPR

Zhou et al. developed a gravity driven SPR sensor. This power-free flow injection system is easy to use and the internal reference compensates for flow fluctuations. The miniature and power-free flow injection system would open the possibility to build a miniature system for point of care testing.

Goto referenence

Gravity SPR

SPR Biosensor based on smart phone platforms

Check this article if you are interested in simple and cheap SPR solutions. The authors show a SPR instrument that makes use of a smart phone to deliver and detect the signal.

Goto article

SPR Phone

Standardized antibodies

Do you use the best reagents? Do you know how your antibodies work?
Ever bought antibodies that did not perform as expected?
Read the comment in Nature and improve your experiments by using validated protein-binding reagents.

Goto article

Nature image

Single surface referencing

Springer et al introduces single surface referencing. A single functionalized surface is split into the detection and reference channel to which complex sample (detection) and complex sample mixed with biomolecules binding to the analyte and thus inhibiting the binding of the analyte to the functionalized surface (reference) is introduced. Goto reference

Single surface referencing

BiaMethodWriter

BiaMethodWriter

Making programming methods easier.
 Info 


Scrubber

Scrubber

This well designed program helps you to clean and analyse your sensorgrams in minutes. Reference subtraction, double referencing, DMSO compensation, almost with one mouse click. Version 2c of Scrubber extends the program with kinetic models for simple binding (association and dissociation rates) and a model for mass transport limited binding.
Scrubber info