Just want to say this website is a fantastic resource, thank you !
I wanted to know the best way to deal with analyte buffer and running buffer discrepancies to get the best I know they should be matched but they can't be buffer exchanged
I am running purified antibody samples from various subjects for kinetic analysis
I run each at 5 concentrations in a serial dilution
Each of my analyte/antibody is at different stock concentration in PBS
I dilute them in my running buffer
Low conc stock analyte means almost 75% of my injection will be PBS for that sample at the highest conc
Will my reference flow cell subtraction take care of this bulk effect?
Or should I run a blank of not running buffer but of the concentration of pbs in running buffer for my highest conc to double ref for each sample?