At the moment I'm working for the first time with the Biotin CAPture chip from GE. It's a very stable chip when you have biotinylated ligands and makes is useful for many cycles without losing signal. However, it seems that in many cases, and especially at high analyte concentrations there is high unspecific binding in the reference cell.
It's manageable by e.g increasing the salt concentration of the buffer. However, the nonspecific binding is never fully gone and depending on the analyte it's high even at low concentrations.
Does anyone of you have the same problem when using the chip? Or does anyone know what's the reason for it? Does the DNA or the streptavidin somehow interact with e.g. antibodies as an analyte?
Also, would you know of some tricks to get rid of it?
Already thanks for reading my post and giving your thoughts about this
Unspecific Binding in Reference Cell of Biotin CAPture chip
11 Aug 2017 19:31 #2