Forum :: What's the situation, disappearing during the SPR experiment?


Welcome, Guest
Username: Password: Remember me
This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: What's the situation, disappearing during the SPR experiment?

What's the situation, disappearing during the SPR experiment? 29 Jun 2017 08:35 #1

Greetings.
actually, when I did experiment serial dilution in SPR.
but... the plot is NOTso good...

hc15d745.jpg

In this plot, curve was extremely fallen at 2^-8(781.3pM), and curve draw zig-zag durong Injection is end.
+DNA was 30 times diluted by half, 30th conc. is 100nM.
I run experiment before, it was good except bulk effect of some area...

DNA_2017-06-29.jpg

(like this) This is good plot except 2^-30~2^-21...

By the way, there's a strange phynomenon. in forst graph, only channel 2 drwas zig-zag, and... look this band.


1.jpg


14400.jpg

The band where middle is channel 2. When I injected sample on first time, the band were vivid each channel. (peristic pump is OK but It notified 'vacuum' frequently) and, look at the band picture below, band of channel 2, half of band disappeared...

Why band dosappears during SPR experiment? and How can I prevent it?

P.S

16567.jpg

The band refreshed half when washing stage(after inject sample).
K.S.Ha
Last Edit: 29 Jun 2017 08:58 by Koreanraichu. Reason: (added something)
Moderators: Arnoud