Forum :: Single Cycle Kinetics


Welcome, Guest
Username: Password: Remember me
This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Single Cycle Kinetics

Single Cycle Kinetics 13 Apr 2017 07:13 #1

Hi Arnoud,
I am seeing a reference flow cell binding when I am trying to do a Single cycle kinetics. I use Biacore T200 and i am trying a capture method.So I immobilise Antihis and capture FcRn. My FC1 has higher bindig than FC2 even for 0 concentration which is the buffer alone.
Please find the attachment for bargraph of FC1.
Need your suggestion.

Thanks
Attachments:

Single Cycle Kinetics 14 Apr 2017 09:28 #2

Hi,

This is strange indeed. I have no direct answer for you. You could try to verify that the injection system is performing alright by doing a system check.

Arnoud
Moderators: Arnoud