Pre-concentration - Forum


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
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TOPIC: Pre-concentration

Pre-concentration 23 Mar 2017 17:14 #1

Hi all,
I'm new to Biacore so any help will be appreciated.
When trying to run the pre-concentration test the sensogram dips down to different levels instead of going up.
I use bog-standard HBS running buffer and IgG 25 ug/ml in Na acetate buffer.
Can anyone please explain?
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Pre-concentration 24 Mar 2017 09:32 #2

Hi,

Strange indeed.
Can you tell which type of sensor chip you use?
And what is the buffer of the IgG?

Arnoud

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Pre-concentration 24 Mar 2017 22:35 #3

Hi,
Thanks for your reply. I'm using standard GEHealthcare CM-5 chip. I was using a custom program, but then used the wizard for pH scouting all worked as it should.
However, when used 2.5 ug/ml and the wizard it went downwards again :(
I'm guessing the concentration in the latter was too low...
Thanks again. T
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