I'm new to Biacore so any help will be appreciated.
When trying to run the pre-concentration test the sensogram dips down to different levels instead of going up.
I use bog-standard HBS running buffer and IgG 25 ug/ml in Na acetate buffer.
Can anyone please explain?
Thanks for your reply. I'm using standard GEHealthcare CM-5 chip. I was using a custom program, but then used the wizard for pH scouting all worked as it should.
However, when used 2.5 ug/ml and the wizard it went downwards again
I'm guessing the concentration in the latter was too low...
Thanks again. T