Forum :: How can I solute reference channel's curve?


Welcome, Guest
Username: Password: Remember me
This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: How can I solute reference channel's curve?

How can I solute reference channel's curve? 23 Jan 2017 03:15 #1

DNA.jpg

(run at 17th Jan)

DNA_2017-01-23.jpg

(run at same day)

Dotted graph is reference channel, and other line is sample. sample channel changes(buffer-sample tube-buffer) when checked equilibrium, but reference don't changes tube or other solution, it keep going with running buffer until experiment end. But as you see above graph, reference channel have a curve... what should I do in this case?
K.S.Ha

How can I solute reference channel's curve? 23 Jan 2017 19:50 #2

I tried to look up the type of instrument you have but could not find it. Do have a reference.
If I understand correctly, the reference is just running buffer only. If you get a peak in the reference, something is wrong with the instrument (e.g. not clean, a bad valve not switching correctly, pump not stable).

You cannot use these curves.
First try to do some dummy experiments with buffer injections until all injections give perfectly flat curve. Then inject buffer with 25 mM extra NaCl to mimic bulk effect. The curves should jump up and get stable during the injection and jump down to baseline after injection. If not check your system.

Kind regards
Arnoud
The following user(s) said Thank You: Koreanraichu

How can I solute reference channel's curve? 24 Jan 2017 02:40 #3

All of progress(streptavidin-linker-sample), in SA and linker, reference curve saw clear, and no get out of range and shake. but it was only in DNA injection,

You're understand correctly, dotted line is reference channel with running buffer only.
K.S.Ha

How can I solute reference channel's curve? 26 Jan 2017 07:27 #4

I did experiment that you advised, reference curve is flat, has no problem.. I think that is buffer's problem.
K.S.Ha
Moderators: Arnoud