Dotted graph is reference channel, and other line is sample. sample channel changes(buffer-sample tube-buffer) when checked equilibrium, but reference don't changes tube or other solution, it keep going with running buffer until experiment end. But as you see above graph, reference channel have a curve... what should I do in this case?
I tried to look up the type of instrument you have but could not find it. Do have a reference.
If I understand correctly, the reference is just running buffer only. If you get a peak in the reference, something is wrong with the instrument (e.g. not clean, a bad valve not switching correctly, pump not stable).
You cannot use these curves.
First try to do some dummy experiments with buffer injections until all injections give perfectly flat curve. Then inject buffer with 25 mM extra NaCl to mimic bulk effect. The curves should jump up and get stable during the injection and jump down to baseline after injection. If not check your system.
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