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TOPIC: negative curve by mismatch

negative curve by mismatch 19 Jan 2017 09:30 #1

  • Koreanraichu
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This analysis was run at 17th Jan(exp 1)

And this analysis was run at 27th Dec(2016).. (exp 2)

As you see, all of curve are strange... Our lab says buffer effect(by conc. Mismatch)
I don't know how I solve this.

Dilution sample in exp 1 : sample 100ul+(10X TBS 100ul+DW 800ul) ;DNA was diluted at DW(dH2O)
Dilution sample in exp 2 : serial diluted at 1X TBS except 1000X stock(total vol. 1ml, triple diluted by 10x)
Then in exp 1, DNA and TBS is 1X, reference buffer is 1X TBS, too(red line). In exp 2, actually, DNA is 0.999X but in reference, TBS is 1X.

Which dilution method is good for negative curve by buffer mismatch? :ohmy:
Prodecessor did by method in exp 2, and she make very tidy result without negative curve, but my co-worker push on method in exp 1.. What shall I do?

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Koreanraichu @ Korea Univ.
born at Seoul, Republic of Korea
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