Forum :: Long spikes before association and after dissociation


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Long spikes before association and after dissociation

Long spikes before association and after dissociation 03 Jan 2017 21:15 #1

Hi,

I ran my samples from 20-200 uM However, I got two spikes(I can term it peaks)before association and after dissociation. I am not sure whether the fit is good or not. SPR machine details: Reichert Tech.
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Long spikes before association and after dissociation 03 Jan 2017 22:20 #2

Hi, welcome to the forum.

I think you cannot make a valid fit with these kind of peaks. Probably something went wrong with the injection or data processing.
You can check the injection system with just one injection with buffer. The curves should be flat, if not, clean the system.

I recommend you look at www.sprpages.nl/sensorgram-tutorial/a-sensorgram for an overview of a proper sensorgram shape.

kind regards

Arnoud
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