Forum :: please help


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: please help

please help 03 Nov 2016 19:56 #1

Hello! I am a new user for BiacoreT200. I did a protein calibration on monday and everydata looks good. But on Tuesday, I did see these signal even with blank buffer injection. I tried desorbing, did not work. Thanks!
TestofHIGG2mgmlinsamplediluentusingoldCM5chipcoatedwithProA.png


TestofHIGGp00133mgmlinsamplediluentusingnewCM5chipcoatedwithProA.png


TetsofsamplediluentblankbufferusingnewCM5chipcoatedwithProA.png

please help 03 Nov 2016 22:24 #2

Hi Dan,

When injecting the buffer, can you check the tubing behind the left door.
Are they all filled without any air bubbles? Check if all the connections are fastened well.

If the spikes persist, you can check the injection needle if there is some dirt on it. Refer to the maintenance manual how to open the sample compartment.

Kind regards

Arnoud

please help 04 Nov 2016 00:52 #3

Hi Arnoud,
I did check the syringe behind the left door. The 500ul one is sooo dirty but not leaky. I have replaced the 500ul syringe and run the desorb again. Will run assay tomorrow morning and let you know the results. Thanks a lot!

Dan
Moderators: Arnoud