I am trying to perform a single cycle kinetics to evaluate an antibody against a toxin. The toxin is immobilized in a CM5 sensorchip by amine coupling. Everything could be right except by the fact that just after the sample injection there is a long spike that happens during a few seconds, which at the end prevents the calculation by Biacore software. I changed the buffer, I did desorb/sanitize and superclean, I prepared another sensorchip... Could anyone tell me what else I should do?
What kind of Biacore do you use? This happens already several times? And why not at the last three injections? I think it is at the start of the injections. Did you check if there is no air in the tubes?