Forum :: Long spike after injection

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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Long spike after injection

Long spike after injection 27 Jun 2016 16:36 #1

I am trying to perform a single cycle kinetics to evaluate an antibody against a toxin. The toxin is immobilized in a CM5 sensorchip by amine coupling. Everything could be right except by the fact that just after the sample injection there is a long spike that happens during a few seconds, which at the end prevents the calculation by Biacore software. I changed the buffer, I did desorb/sanitize and superclean, I prepared another sensorchip... Could anyone tell me what else I should do?

An example of a sensorgram:


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Long spike after injection 27 Jun 2016 16:59 #2


What kind of Biacore do you use? This happens already several times? And why not at the last three injections? I think it is at the start of the injections. Did you check if there is no air in the tubes?

Kind regards


Long spike after injection 27 Jun 2016 21:01 #3

Hi, I am using Biacore T200.
Yes, it happens most but not all the runs. I thought it could be air bubbles, but this means I may have equipment problems...

Thank you

Long spike after injection 27 Jun 2016 21:09 #4

What you can check is the pumps, behind the door.
If you trigger a prime you can see if the pumps are fully filled while operating.
If they draw some air you know you need some servicing.

The following user(s) said Thank You: wagquint

Long spike after injection 01 Jul 2016 20:51 #5

I called GE service. They tightened the degasser connectors and advised me to perform "Flush system wash". I did it twice and the problem seems solved.
Thank you again! B)
Last Edit: 01 Jul 2016 20:55 by wagquint.
The following user(s) said Thank You: Arnoud, JeffH
Moderators: Arnoud