Forum :: Long spike after injection


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Long spike after injection

Long spike after injection 27 Jun 2016 16:36 #1

I am trying to perform a single cycle kinetics to evaluate an antibody against a toxin. The toxin is immobilized in a CM5 sensorchip by amine coupling. Everything could be right except by the fact that just after the sample injection there is a long spike that happens during a few seconds, which at the end prevents the calculation by Biacore software. I changed the buffer, I did desorb/sanitize and superclean, I prepared another sensorchip... Could anyone tell me what else I should do?

An example of a sensorgram:

sensorgram1.png


Zoom in:

sensorgram2.png

Long spike after injection 27 Jun 2016 16:59 #2

Hi,

What kind of Biacore do you use? This happens already several times? And why not at the last three injections? I think it is at the start of the injections. Did you check if there is no air in the tubes?

Kind regards

Arnoud

Long spike after injection 27 Jun 2016 21:01 #3

Hi, I am using Biacore T200.
Yes, it happens most but not all the runs. I thought it could be air bubbles, but this means I may have equipment problems...

Thank you

Long spike after injection 27 Jun 2016 21:09 #4

What you can check is the pumps, behind the door.
If you trigger a prime you can see if the pumps are fully filled while operating.
If they draw some air you know you need some servicing.

Arnoud
The following user(s) said Thank You: wagquint

Long spike after injection 01 Jul 2016 20:51 #5

Hi!
I called GE service. They tightened the degasser connectors and advised me to perform "Flush system wash". I did it twice and the problem seems solved.
Thank you again! B)
Last Edit: 01 Jul 2016 20:55 by wagquint.
The following user(s) said Thank You: Arnoud, JeffH
Moderators: Arnoud