Forum :: SPR validation

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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: SPR validation

SPR validation 15 Mar 2016 18:49 #1

I am looking for papers in which data obtained with SPR are validated with data obtained with a different technique that does not immobilises the receptor. In particular I am interested in albumin binding but also other topics could be of interest.
Many thanks


SPR validation 15 Mar 2016 20:25 #2

Hi Giulia,

A quick search gave me these two references.

1. Day, Y. S., Baird, C. L., Rich, R. L., et al.; Direct comparison of binding equilibrium, thermodynamic, and rate constants determined by surface- and solution-based biophysical methods. Protein Science (11) 5: 1017-1025; 2002.
2. Navratilova, I., Papalia, G. A., Rich, R. L., et al.; Thermodynamic benchmark study using Biacore technology. Analytical Biochemistry (364) 1: 67-77; 2007.

Kind regards


SPR validation 16 Mar 2016 10:31 #3

Many thanks. I am looking for more recent papers like 1. In particular, it would be fine to verify the correspondence between surface- and solution-based biophysical methods for receptors different from carbonic anydrase.

SPR validation 18 Mar 2016 20:20 #4

Hi Giulia

These are more recent. Maybe they wll help you further

1. Groftehauge, M. K., Hijizadeh, N. R., Swann, M. J., et al.; Protein-ligand interactions investigated by thermal shift assays (TSA) and dual polarization interferometry (DPI). Acta Crystallographica Section D Biological Crystallography (71) 1: 36-44; 2014.
2. Mondal, K., Regnstrom, K., Morishige, W., et al.; Thermodynamic and kinetic characterization of hydroxyethylamine β-secretase-1 inhibitors. Biochemical and Biophysical Research Communications (441) 2: 291-296; 2013.
3. Winquist, J., Geschwindner, S., Xue, Y., et al.; Identification of Structural–Kinetic and Structural–Thermodynamic Relationships for Thrombin Inhibitors. Biochemistry (52) 4: 613-626; 2013.

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