Forum :: Streptavidin Chip


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Streptavidin Chip

Streptavidin Chip 23 Feb 2016 00:43 #1

Do I need a reference channel in case of using streptavidin chip. And if so what exactly I should use in this reference channel?

Thanks

Streptavidin Chip 24 Feb 2016 08:31 #2

Hi Ahmed,

It is better to have a reference channel to compensate for small buffer differences, drift and non-specific binding to the matrix.

The best reference is a related molecule that will not bind the analyte.
When that is not available, use a non related molecule of the same size.
When creating a refrence channel try to match the loading of the ligand with that of the protein of interrest.
For the SA-chip you can think of blocking the streptavidin with biotin to create a 'neutral' surface.

Kind regards

Arnoud
The following user(s) said Thank You: ahmedaboraia

Streptavidin Chip 29 Feb 2016 20:46 #3

Thanks a lot Arnoud
Moderators: Arnoud