Sensorgram increase at the start - Forum


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TOPIC: Sensorgram increase at the start

Sensorgram increase at the start 04 Jan 2016 21:43 #1

  • ahmedaboraia
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Dear all,

Can anybody give me his opinion in the reason why my sensorgram shows a small increase after injection which is followed by small decrease and completion of association and dissociation phases afterward (as shown in the attached figure specially
with the lower conc)?

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Sensorgram increase at the start 05 Jan 2016 10:21 #2

Hi Ahmed,

The spike come from the fact that the channels are in series. When subtracting a reference, both channels are a little out of phase.
In the Biacore you can use the direct referencing during the experiment which will give less spikes.
Or you can adjust the injection time point t=0 for each curve.

When using a program like Scrubber, you kan artificially remove the spikes.

Large spikes occur mainly when the bulk effect is large (>25 RU) and the compensation by the reference channel is not optimal.

Kind regards

Arnoud

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