Forum :: Random Spikes during run/ immobilization


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Random Spikes during run/ immobilization

Random Spikes during run/ immobilization 16 Oct 2015 09:33 #1

T100spikes.jpg

Hi all/arnound,

I am getting these spikes randomly during the run and sometimes during immobilization. I am attaching an image of a spike during the kinetic run. it is not specific to a particular interaction... it is very random. i am using Biacore T100 and the system is well maintained with Desorb and sanitize and only desorb as per recommendation
Last Edit: 16 Oct 2015 09:43 by biaenthu.

Random Spikes during run/ immobilization 16 Oct 2015 21:18 #2

Hi,

What you see is probably an air spike although it should not happen within a T100. I trust you filter your buffers to get out small particles like dust. You can degass the buffer yourself and look if there are less spikes.
Maybe the injection port needs cleaning (look in the book).

regards

Arnoud
Moderators: Arnoud