Double peak - Forum


Welcome, Guest
Username: Password: Remember me
This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
  • Page:
  • 1

TOPIC: Double peak

Double peak 23 Jul 2015 09:28 #1

  • avsurround
  • avsurround's Avatar
    Topic Author
  • Offline
  • Posts: 2
Hello,

I've been doing some fragment library screening using 12.5k RU of immobilized protein and 1 mM fragments in 2% DMSO. The majority of sensorgrams are looking quite good except for some that exhibit a double peak. Where could the problem be?

Too much protein? Too high concentration of analyte? Fragment-structure dependent? Flow rate not fast enough?

Thanks!


Attachments:

Please Log in or Create an account to join the conversation.

Double peak 24 Jul 2015 10:12 #2

Hi,

Speculation: maybe the mixing of the analyte in the vial? Possible aggregation of analyte?

Or just bad luck.

Kind regards

Arnoud

Please Log in or Create an account to join the conversation.

  • Page:
  • 1
Moderators: Arnoud