Forum :: Double peak


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Double peak

Double peak 23 Jul 2015 09:28 #1

Hello,

I've been doing some fragment library screening using 12.5k RU of immobilized protein and 1 mM fragments in 2% DMSO. The majority of sensorgrams are looking quite good except for some that exhibit a double peak. Where could the problem be?

Too much protein? Too high concentration of analyte? Fragment-structure dependent? Flow rate not fast enough?

Thanks!


doublepeak.jpg

Double peak 24 Jul 2015 10:12 #2

Hi,

Speculation: maybe the mixing of the analyte in the vial? Possible aggregation of analyte?

Or just bad luck.

Kind regards

Arnoud
Moderators: Arnoud