Low response during secondary binding using NHS/EDC - Forum


Welcome, Guest
Username: Password: Remember me
This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
  • Page:
  • 1

TOPIC: Low response during secondary binding using NHS/EDC

Low response during secondary binding using NHS/EDC 23 Apr 2015 21:41 #1

Hi, I'm using homemade SPR system. I have a general question on surface functionalization that somebody might have similar experience and solution: I'm trying to do antigen-antibody capture using NHS/EDC . I saw a response during the binding of antigen on NHS/EDC modified carboxylated surface but I have a negative response when I inject the corresponding antibody. The antigen and antibody pair are known to react with each other. I obtained a response for these antigen-antibody before and they just suddenly stopped working. Any guess on what is wrong with the chemistry? Thank you.

Please Log in or Create an account to join the conversation.

Low response during secondary binding using NHS/EDC 28 Apr 2015 21:07 #2

Hi,

Please look at www.sprpages.nl/experiments/troubleshooting.html for some trouble shooting.

Please Log in or Create an account to join the conversation.

  • Page:
  • 1
Moderators: Arnoud