Forum :: Low response during secondary binding using NHS/EDC


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: Low response during secondary binding using NHS/EDC

Low response during secondary binding using NHS/EDC 23 Apr 2015 21:41 #1

Hi, I'm using homemade SPR system. I have a general question on surface functionalization that somebody might have similar experience and solution: I'm trying to do antigen-antibody capture using NHS/EDC . I saw a response during the binding of antigen on NHS/EDC modified carboxylated surface but I have a negative response when I inject the corresponding antibody. The antigen and antibody pair are known to react with each other. I obtained a response for these antigen-antibody before and they just suddenly stopped working. Any guess on what is wrong with the chemistry? Thank you.

Low response during secondary binding using NHS/EDC 28 Apr 2015 21:07 #2

Hi,

Please look at www.sprpages.nl/experiments/troubleshooting.html for some trouble shooting.
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