Forum :: immobilization/ amine coupling


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This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.

TOPIC: immobilization/ amine coupling

immobilization/ amine coupling 19 Feb 2014 08:47 #1

Hi,

I would like to immobilize a basic protein (Theoretical pI: 9,53) by amine coupling to sensor CM5 or CM4. I am afraid that immobilization in recommended conditions (pH 6.0) will lead to inactivation of the protein. What do you think about amine coupling in more physiological conditions (about pH 7.4)?
I wouldn’t like to use SA sensor.

Thank you.
Cotix
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