Forum :: calibration drift/instability


Welcome, Guest
Username: Password: Remember me
These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: calibration drift/instability

calibration drift/instability 20 May 2005 02:00 #1

I would like to hear if anyone has some suggestions for the following problem. I am
currently trying to set up a conc. measurement method using a CM5 chip. Analyte is
55 kDa, the ligand 50 kDa. The chip is immobilized with 2000 RU. The problem is that
ex. 4 calibration curves in series suffer from drift/instability (the data points do
not lay close to a average curve!). Regeneration is fine and there is not baseline
drift. I tend to think that I am having some conformational change problems with the
immobilized ligand. How do I stabilize the ligand - another reg. solution, washing
after regeneration?

Let me here some suggestions?
Michael

calibration drift/instability 20 May 2005 02:00 #2

First step is to read the pages on

<Getting the best results>

In <Experimental artifacts> pay special attention to

- Analyte concentration
- Steric hindrance
- Independent vs linked reactions

I hope you get some clues to solve ypur problem.

Arnoud