Forum :: NSB reducer


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: NSB reducer

NSB reducer 19 Sep 2011 02:00 #1

Hello. Has anyone used the NSB reducer from Biacore (10mg/mL of carboxymethyl dextran in 0.15M NaCL)? It said to use a 1:10 diln with the samples before injection (comes in a 10mg/mL solution). How about the buffer? Shouldn't that contain CM dextran as well to match sample and buffer matrix? I was thinking of just preparing the HBS buffer and add 1mg/mL of CM-dextran (powder), filter and then use this buffer to prepare the samples. Kindly comment. Thanks.
mtarca

NSB reducer 19 Sep 2011 02:00 #2

Hi mtarca,

I would add it to the flow buffer to make the buffer jumps lower.
I think you should use the lowest concentration which is working for you.

In addition, maybe you should try to identify the source of the non-specific binding.

Arnoud