Forum :: Tannin binding


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Tannin binding

Tannin binding 01 Sep 2011 02:00 #1

Hi,
I'm binding procyanidins (Tannin) to the gold surface, but no success in removing. I've tried with Urea 6M, NaOH 0,1M-Triton 1%, Hydrogen Peroxide. Any suggestions?
John

Tannin binding 01 Sep 2011 02:00 #2

Hi this not be easy because gold adsorbs proteins and other stuff very easy.

Try 4% SDS.

The mixture of 0.1 M NaOH and 1% Triton X-100 should work in most cases but maybe is your protein sticky.

Look at the following link (www.ti.com/lit/an/slya018a/slya018a.pdf) for more information and hints.