Tannin binding - Forum


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TOPIC: Tannin binding

Tannin binding 01 Sep 2011 02:00 #1

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Hi,
I'm binding procyanidins (Tannin) to the gold surface, but no success in removing. I've tried with Urea 6M, NaOH 0,1M-Triton 1%, Hydrogen Peroxide. Any suggestions?
John

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Tannin binding 01 Sep 2011 02:00 #2

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Hi this not be easy because gold adsorbs proteins and other stuff very easy.

Try 4% SDS.

The mixture of 0.1 M NaOH and 1% Triton X-100 should work in most cases but maybe is your protein sticky.

Look at the following link ( www.ti.com/lit/an/slya018a/slya018a.pdf ) for more information and hints.

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