Forum :: non specific binding issues


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: non specific binding issues

non specific binding issues 05 Jun 2011 02:00 #1

anyone, we think some non-specific binding is occuring and what to try out adding more P20 surfactant to the existing HBS-P buffer but not sure how much to add. any suggestions? currently the HBS-P has 0.5% v/v of P20 in it. Also would adding BSA to samples help? Any suggestions would be most helpful.....Thanks.
mtaraca

non specific binding issues 05 Jun 2011 02:00 #2

0.5% is alrealdy quite a lot. You could try adding BSA to the running buffer (say 0.5 mg/ml for a start) and some extra salt (0.5M) or dextran. Working at low concentrations can also help.
joseantonio