RU increase upon injection finish - Forum


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
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TOPIC: RU increase upon injection finish

RU increase upon injection finish 08 Apr 2011 02:00 #1

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Hi James,

I can see the picture in your profile, but would it be possible to see both channels separately?

Regards,

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RU increase upon injection finish 08 Apr 2011 02:00 #2

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You are sure it is not a bulk RI change when going back to running buffer? This would agree with the concentration dependence (if the jump is proportional). But I guess that you also referencing this, right?
joseantonio

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RU increase upon injection finish 08 Apr 2011 02:00 #3

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Cheers for the response joseantonio.

Aye, the change in RU seems to exceed the RI change between sample and running buffers. I hope its not a fluidics issue, perhaps it is due to the lack of surfactant (P20) in running buffer?
james

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RU increase upon injection finish 08 Apr 2011 02:00 #4

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You should definitely add surfactant to your running buffer. Can you tell the bulk RI change from that other RI change you mention? If so, how do you do that? Have you tried injecting just running buffer?

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RU increase upon injection finish 08 Apr 2011 02:00 #5

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Problem is P20 prevents the interaction between my analyte and ligand. As it goes I am using it for Regeneration solution (it works a treat on hydrophobic interactions). The dips either side of the injection result from the delay between RI changes in FC1 and FC2 and they match up in terms of RU change. The raise in RU that I'm seeing post injection is considerably more though. But yes we also injected running buffer only as well, which correlated nicely. I wonder if this issue might be caused by adsorbed aggregates in the IFC?

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RU increase upon injection finish 08 Apr 2011 02:00 #6

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I apologize but I dont the get picture. Could you post a sketch (made in paint) or a screenshot of the response in FC1 and FC2)?

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