Forum :: Chip preconditioning


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Chip preconditioning

Chip preconditioning 31 Mar 2011 02:00 #1

Is it necessary to run these injections (sodium hydroxide, hydrochloric acid, SDS and/or phosphoric acid) to precondition a C1 chip? Or is it just necessary/recommended for dextran chips?

Thanks,
joseantonio

Chip preconditioning 31 Mar 2011 02:00 #2

i've just open a c1 chip, they say to injected with a solution prepared with 0.1M glycine-NaOH pH 12 with 0.3% triton buffer.! then do a prime with the running buffer

cheers
pierqiuseppe

Chip preconditioning 31 Mar 2011 02:00 #3

Thanks. I am using my own chips, but they are very like a C1 chip. I will include that injections in my routines. BTW, I build my SAMs with MUA, but does anybody knows how they prepare the C1 chips exactly? It is just curiosity.
joseantonio