Forum :: DNA-SH immobilisation


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: DNA-SH immobilisation

DNA-SH immobilisation 30 Mar 2011 02:00 #1

could anyone indicatea protocol for DNA-SH immo onto gold surface ?

thanks

dd

DNA-SH immobilisation 30 Mar 2011 02:00 #2

Why would you want to immobilize the SH-DNA directly to the gold surface?
Ik think that using a dextran coated chip and a thiol immobilizing protocol will be more efficient.

Arnoud

DNA-SH immobilisation 30 Mar 2011 02:00 #3

Just dilute the thiolated DNA (~1uM) in a buffer (I use PBS) and incubate it over a couple of hours. Then, if you want, do an extra half hour long incubation of MCH (1mM) in the dark.