Hi, I'm trying to use the GE healthcare GST capture kit with a CM5 chip
The kit has anti-GST and immobilisation buffer and I'm trying to NHS-EDC couple the anti-GST to the chip
The kit estimated I should be getting 7500 RU bound. This allows me to then couple about 500 RU of GST-fusion to this to then bind to my analyte
Problem is, I can see a really big peak when I flow the anti-GST over - around +30000 RU
But most of this doesn't leaving me with about 500RU on the chip.
Not sure what the problem is, I've tried lower flow rate and high  of the anti-GST