My lab will be acquiring a BIAcore X machine for a short period of time on a lease agreement and we were wondering about the requirements for setup (as we need to consider a limited budget):
A : Do we need to purchase all the acetate and borate buffers for preconcentration or can we make our own?
B : Does the BIAcore X machine require a pressurised gas canister?
C : Can we make our own regeneration buffers?
D : How many times can one use the NTA and Streptavidin chips?