Biacore X and requirements for setup - Forum


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.
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TOPIC: Biacore X and requirements for setup

Biacore X and requirements for setup 15 Apr 2009 02:00 #1

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Hi
My lab will be acquiring a BIAcore X machine for a short period of time on a lease agreement and we were wondering about the requirements for setup (as we need to consider a limited budget):
A : Do we need to purchase all the acetate and borate buffers for preconcentration or can we make our own?
B : Does the BIAcore X machine require a pressurised gas canister?
C : Can we make our own regeneration buffers?
D : How many times can one use the NTA and Streptavidin chips?
Thanks
Earl

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Biacore X and requirements for setup 15 Apr 2009 02:00 #2

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A: it is possible to make your own. Use degassed water and high grade chemicals. Filter after prepearation. Store in aliqouts at -20C for long term.

B: I'am not familiar with the biacore X but i thought not.

C: Yes, just like A

D: this realy depends on what you do. NTA you can regenerate but with the SA-chip it is very hard to strip of bound proteins due to the nature of the SA-biotin binding.

Youre welcome

Arnoud

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Biacore X and requirements for setup 15 Apr 2009 02:00 #3

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Perfect,
Thanks for the speedy reply. This will cut our budget and allow us to purchase more chips hopefully.
Regards,
Earl

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