Forum :: SPR interaction for small molecules


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: SPR interaction for small molecules

hydrophobic 05 Mar 2009 01:00 #1

Did you modify the reference or is it native carboxyl and hydroxyl groups?
Which type/brand of chip are u using?

It can be beneficial to activate and block the reference with ethanolamine of other small amine components.

hydrophobic 05 Mar 2009 01:00 #2

Thanks for your comment, Well, I did EDC/NHS activation on channel 1(in which I immobilize the protein) and did ethanol amine capping on both channels. I used, SensiQ gold only chips from icx nomadics. SAM was prepared by myself.
Do I need to do EDC/NHS activation on both channels?

hydrophobic 05 Mar 2009 01:00 #3

The activation does what it say. Activation of the sensor surface so it can bind other molecules. In case of the NHS/EDC the carboxyl groups of your SAM are modified so that the next injection with primary amines (such as ethanol amine) will bind. Without activation no immobilization.

I suggest you look at www.sprpages.nl/immobilization.htm for a detailed description.
Last Edit: 13 Mar 2015 22:50 by MQAdmin03.

Activation/deactivation 05 Mar 2009 01:00 #4

Thanks for your comments. So far I have been activating with EDC/NHS only in channel one (where I Immobilize the protein) and I did the ethanol amine injection on both channels. I guess as per your comments, I have to activate both channels ( including reference channel ) with EDC/NHS and then de-activate with ethanol amine on both channels right? Let me do in this way and see what happens.
Preejith SLC

SPR interaction for small molecules 17 Dec 2013 21:01 #5

I am trying to use SPR to study the interaction of small molecules ( my compounds are hydrophobic and sticky) with its binding protein. I am getting too much of nonspecific binging on the reference channel, so its hard to extract the specific binding. I used a mixed carboxyl and hydroxyl SAM to bind the protein Does anyone got experience with these kinds of molecule interaction using SPR?
Preej