Forum :: Biotin-tagged molecule immobilisation

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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Biotin-tagged molecule immobilisation

Biotin-tagged molecule immobilisation 30 Jan 2008 01:00 #1

Hi all,

I have read that successful biotin-tagged molecules immobilization need to reach a stable plateau.
Is it possible to reach a stable plateau also if you want a low immobilization level?

Biotin-tagged molecule immobilisation 30 Jan 2008 01:00 #2

I'am not sure I understand your question.

The avidin-biotin binding is a very strong binding. The binding is strong enough to be considered stable for your purposes.

When binding your biotin-tagged protein to a avidin sensor surface the response after immobilization must be stable even if you have a low immobilization level.

You can block the remaining avidin sites with a biotin injection and stabilize the surface with some high salt pH 8.5 injections (if possible with yor ligand).


Biotin-tagged molecule immobilisation 30 Jan 2008 01:00 #3

Thanks for your reply Arnoud,

Do you have access to BIAapplications Handbook from Biacore? At the end of the Streptavidin chapter (Page 4-26) it says that successful binding to SA surface means that the immobilization curve reach a stable plateau.