Forum :: Global or single fitting ?

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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Global or single fitting ?

Global or single fitting ? 15 Sep 2004 02:00 #1

How is better to interpret kinetic data? Is it more realable a
global and simultaneous fittiong of the curves or a separate one, curve by
curve? I tried to use both but I obtained very different results.

Global or single fitting ? 15 Sep 2004 02:00 #2

Hi Ilaria

I would say that a global fit is better than a fit obtained by separatly fitted curves of different concentrations. Another advantage is that you need less time for the analysis.
What you can do is to compare KD's calculated from the obtained kon,koff with the one determined by equilibrium analysis (e.g. by Scrubber).
This may validate one of the methods.