hopefully somone can help us out on the exact procedure for a surface thiol coupling.
we more or less have a protocol, but it lacks info on the solvent of the ligand.
should it be similar to the buffer we applied in the aminocoupling (Naacetate; pH 4.0)?
any more tips are very welcome.
Reversible Surface Thiol Immobilization of Carboxyl Group
Containing Haptens to a BIAcore Biosensor Chip Enabling Repeated Usage of a Single Sensor Surface
Ulrich Schlecht, Yoko Nomura, Till Bachmann, and Isao Karube*
Bioconjugate Chem. 2002, 13, 188-193