Forum :: Thiol coupling


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Thiol coupling

Thiol coupling 05 Oct 2007 02:00 #1

Hi,

hopefully somone can help us out on the exact procedure for a surface thiol coupling.
we more or less have a protocol, but it lacks info on the solvent of the ligand.
should it be similar to the buffer we applied in the aminocoupling (Naacetate; pH 4.0)?
any more tips are very welcome.

thanks,
Jip

Thiol coupling 05 Oct 2007 02:00 #2

This reference may be of help

Reversible Surface Thiol Immobilization of Carboxyl Group
Containing Haptens to a BIAcore Biosensor Chip Enabling Repeated Usage of a Single Sensor Surface
Ulrich Schlecht, Yoko Nomura, Till Bachmann, and Isao Karube*
Bioconjugate Chem. 2002, 13, 188-193

Thiol coupling 05 Oct 2007 02:00 #3

Is it possible to store PDEA-modified protein in the freezer -20oC?

And how long does it remain stable?

Thanks for helping.

Thiol coupling 05 Oct 2007 02:00 #4

PDEA modified protein can be stored at -20C if the protein tolerates the freezing. In my case, the PDEA-modified protein did not lose any immobilization capacity within 2 month.

Strada