Thiol coupling - Forum


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TOPIC: Thiol coupling

Thiol coupling 05 Oct 2007 02:00 #1

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Hi,

hopefully somone can help us out on the exact procedure for a surface thiol coupling.
we more or less have a protocol, but it lacks info on the solvent of the ligand.
should it be similar to the buffer we applied in the aminocoupling (Naacetate; pH 4.0)?
any more tips are very welcome.

thanks,
Jip

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Thiol coupling 05 Oct 2007 02:00 #2

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This reference may be of help

Reversible Surface Thiol Immobilization of Carboxyl Group
Containing Haptens to a BIAcore Biosensor Chip Enabling Repeated Usage of a Single Sensor Surface
Ulrich Schlecht, Yoko Nomura, Till Bachmann, and Isao Karube*
Bioconjugate Chem. 2002, 13, 188-193

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Thiol coupling 05 Oct 2007 02:00 #3

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Is it possible to store PDEA-modified protein in the freezer -20oC?

And how long does it remain stable?

Thanks for helping.

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Thiol coupling 05 Oct 2007 02:00 #4

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PDEA modified protein can be stored at -20C if the protein tolerates the freezing. In my case, the PDEA-modified protein did not lose any immobilization capacity within 2 month.

Strada

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