I'm just about to do my first SPR experiment (on Monday), but the method was only briefly introduced to me. And so, one important question arose: what kind of buffer may I use as the flow buffer?
Few things: I wanted to use sodium acetate for ligand that's going to be immobilised. Then, my sample is in the Tris buffer. And my activating solution is in MES. Now, can I use the acetate as my flow buffer? Or shall I rather use Tris? And - in any case - won't these buffers interfere with the immobilisation?
Reading - that's what I'm doing since more than a week. And believe me - I've already read through all the stuff from that page. It might only be that I haven't understood something.
Anyway, I just thought that it would be better if the flow buffer was the same as one of the others buffers I'd like to use - or have to use, because I can't help e.g. having my sample in Tris. But it seems that none of them is really apropriate for NHS/EDC coupling, so I suppose I'll have to stick to PBS. Hope it will work.
It will be what it from the beginning had to be - try and error research.