Forum :: Can Sulfo-NHS displace NHS?


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: Can Sulfo-NHS displace NHS?

Can Sulfo-NHS displace NHS? 06 Feb 2007 01:00 #1

Sulfo-NHS is used in crosslink between nanoparticle and target protein, can it also be used in the amine coupling of biacore experiments?

EDC/MHS 06 Feb 2007 01:00 #2

I also wonder how long can I use the prepared EDC and NHS in Amine Coupling Kit of biacore company.The storage time was always short when I got it. It's bad when use old EDC/NHS? And what's the best storage condition for them?

Thank you!

EDC/MHS 06 Feb 2007 01:00 #3

I normally go to Pierce with this kind of questions. They are great in their explanation. Look at:

www.piercenet.com/Product...D=02040114

Arnoud

EDC/MHS 06 Feb 2007 01:00 #4

Thank Arnoud very much!
In some biosensor experiments, some user suggested to prepare NHS and EDAC before amine coupling.They won't use it after 24h. How do you store them? I stored them separately little volume at -20C before. Is it right?

EDC/MHS 06 Feb 2007 01:00 #5

I usually make separate aliquots of NHS and EDC enough for four immobilizations like the biacore protocol for amine coupling.
I store the aliqouts at -20 degrees for up to one year. This seems to work for NHS and EDC.
In addition I do the same for the ethanolamine.

Arnoud