Forum :: why thiol coupling won't work for oligonucleoti


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TOPIC: why thiol coupling won't work for oligonucleoti

why thiol coupling won't work for oligonucleoti 10 Jan 2007 01:00 #1

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Anybody knows why we can not immbolize oligonucleotide through thiol coupling method onto Biacore C1 sensor chip. Thanks for you reply.

Thiol immobilisation 10 Jan 2007 01:00 #2

Are you aware that peptides can give very low signals when immobilized? But you can also easily immobilize to much and mask any further binding!

May this publication can give anay help

1: J Mol Recognit. 2006 Jul-Aug;19(4):363-71. Links
Modular, self-assembling peptide linkers for stable and regenerable carbon nanotube biosensor interfaces.Contarino MR, Sergi M, Harrington AE, Lazareck A, Xu J, Chaiken I.