Forum :: advantages of SPR over FRET


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These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

TOPIC: advantages of SPR over FRET

advantages of SPR over FRET 10 Nov 2006 01:00 #1

Hi All,

I am planning to do some protein: protein interactions. I would like to which technique is better FRET or SPR> Can anyone let me know.

Regards,

Karthik

advantages of SPR over FRET 10 Nov 2006 01:00 #2

I noticed that with FRET the proteins must be labelled in some way. In SPR this is not necessary.

With SPR, small molecules ( < 500 KD) are hard to detect and with FRET this is possible.

Which equipment do you have since both need special machines?

advantages of SPR over FRET 10 Nov 2006 01:00 #3

Thanks Arnoud for your reply. Probably we might use Confocal Microscopy ( zeiss or Olympus) for FRET and for SPR ( Biacore or Reichert). Can you let me know which one is better Your suggestions would be valuable. I am new to this area of work.

advantages of SPR over FRET 10 Nov 2006 01:00 #4

For the SPR I should use the Biacore machine since it comes with ready to use sensorchips in stead of the Reichert (thats what i read in their brochure).