advantages of SPR over FRET - Forum


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TOPIC: advantages of SPR over FRET

advantages of SPR over FRET 10 Nov 2006 01:00 #1

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Hi All,

I am planning to do some protein: protein interactions. I would like to which technique is better FRET or SPR> Can anyone let me know.

Regards,

Karthik

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advantages of SPR over FRET 10 Nov 2006 01:00 #2

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I noticed that with FRET the proteins must be labelled in some way. In SPR this is not necessary.

With SPR, small molecules ( < 500 KD) are hard to detect and with FRET this is possible.

Which equipment do you have since both need special machines?

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advantages of SPR over FRET 10 Nov 2006 01:00 #3

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Thanks Arnoud for your reply. Probably we might use Confocal Microscopy ( zeiss or Olympus) for FRET and for SPR ( Biacore or Reichert). Can you let me know which one is better Your suggestions would be valuable. I am new to this area of work.

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advantages of SPR over FRET 10 Nov 2006 01:00 #4

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For the SPR I should use the Biacore machine since it comes with ready to use sensorchips in stead of the Reichert (thats what i read in their brochure).

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