Thanks for your reply. i will try your suggestions. However i still think it could be easier to inject 60ul of Pronase onto the chip and then undock the chip and leave it overnight. TheBIacore machine could easily be cleaned by using NaOH. However I cannot work out how to inject a solution onto the chip and then leave it there. I suspect that BIAcore has designed the software so that people cannot do this as it would be easier to regenerate the chip this way. I mainly want to use the renerated chip as a screening chip. When I have my buffer conditions correct i would then use a new chip.
Well, the amino coupling is a covalent immobilization method so, I am afraid you should break the covalent bond in order to eliminate the ligand from the surface. Then, it is considered an irreversible immobilization method and you have to use a new flow cell for a new experiment.
Please look at the Download - Howto section en lookup de document about 'How to optimize regeneration'.
In here you will find a protocol to strip de your ligand from the sensorchip and to recodition the sensorchip surface.
I read the paper. To regenerate the chip you have to remove it from the casing and soak in Pronase solution to cleave protein from the dextran layer. A better way would be to inject Pronase onto the chip, however i cannot work out how to do this using the BIAcore software. Does anyone know how i could inject 30ul of Pronase solution into the BIAcore chip .??
Thanks for your reply. I am new to this. I was worried about damaging the chip if i removed it from the casing. Also I was worried that if i damaged the chip, the damaged chip could cause damage to the BIAcore optical detection system. It would be much easier to inject 60ul of Pronase onto the chip and leave it there overnight and then remove using NaOH. If NaOH was injected this would remove any Pronase.