I have a huge technical problem. all in a sudden (after having injected fibronectine) I can't inject anymore or extremely slowly in my Biacore X. I tried all the unclogging; desorption; injection of NaOH , SDS and H3PO4 but nothing seems to help.
Moreover I had prepared thiolated surfaces, which I can't use now. Anyone knows the stability and how can I store them in the meanwhile?
Thanx a lot!