Forum :: ligand immobilization


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TOPIC: ligand immobilization

ligand immobilization 21 Jun 2006 02:00 #1

hi everyone
E 2645 OF SIGMA
Storage Temperature −20 C
This product is soluble in 10% glycerol (50 mg/ml).
Reconstitution with 0.1 ml of 10% glycerol yields a
solution in 50 mM HEPES, pH 7.6, 150 mM NaCl,
0.05% TRITON X-100, 1 mM DTT, and 10%
trehalose.
Then stored at 70 C.
it is recommendable to be used on ice,
can this product be used as ligand to be immobilized on sensor chip? will it be unstable in the operation of biacore?

thank you a lot in advance

ligand immobilization 21 Jun 2006 02:00 #2

In general proteins are relatively stable on the sensorchip but I don't know the ins and outs of this one.
As you probably know 10% glycerol will mask all your responses so you should get rid of it.

I found a reference which may be of help:

PNAS | January 27, 2004 | vol. 101 | no. 4 | 929-934
BIOCHEMISTRY
A structure-based model for ligand binding and dimerization of EGF receptors
Peter Klein * , Dawn Mattoon *, Mark A. Lemmon , and Joseph Schlessinger

Arnoud

ligand immobilization 21 Jun 2006 02:00 #3

Thank you for helping me with my question.I am glad of it! but i can not find the article you mentioned.
Please give me the article if it is convenient to you. Thank you for your kindness!
my e-mail is:guoxiang811@hotmail.com

ligand immobilization 21 Jun 2006 02:00 #4

thank you for your kindness!
i can not find the article you mentioned.

could you send it to me at your convenience.


I'm truly grateful for your help.thanks a lot!
my e-mail address:guoxiang811@hotmail.com


looking forward to your reply soon!